{"id":457,"date":"2013-11-04T09:00:55","date_gmt":"2013-11-04T14:00:55","guid":{"rendered":"http:\/\/www.axopub.com\/wp01\/?p=457"},"modified":"2013-10-31T16:57:26","modified_gmt":"2013-10-31T20:57:26","slug":"warming-up-with-brown-fat","status":"publish","type":"post","link":"https:\/\/www.axopub.com\/wp01\/2013\/11\/04\/warming-up-with-brown-fat\/","title":{"rendered":"Warming up with brown fat."},"content":{"rendered":"
\"Thermographs<\/a>

Thermographs showing body temperature of mice in the day and at night.<\/p><\/div>\n

The paper<\/em><\/strong>: Gerhart-Hines, Z. et al. (2013) The nuclear receptor Rev-erb\u03b1 controls circadian thermogenic plasticity. Nature<\/em>. doi:10.1038\/nature12642<\/p>\n

Subject areas<\/strong><\/em>: Physiology, Cell Biology<\/p>\n

Vocabulary<\/strong><\/em>:<\/p>\n

Zeitgeber Time (ZT) – ZT is a convention used in chronobiology to standardize time effects on biological processes. In the experiments described here, the mice are acclimated to a 24 hour light-dark cycle: lights on at 07:00, off at 19:00 normal time, corresponding to ZT 00:00 to 12:00. The term comes from German chronobiologist Jurgen Aschoff. Zeit means time in German; geber means giver.<\/p>\n

exogenous – caused by or derived from factors outside of a system or organism. \u00a0opposite of endogenous, which in biology usually means something generated by an organism\/cell\/tissue itself.<\/p>\n

—–
\nThis article is a summary of a recent primary research paper intended for high school teachers to add to their general knowledge of current biology, or to supplement their lessons by showing students the kinds of projects that current biological research addresses.
\n—–<\/p>\n

Most of us have been taught since we were little kids that mammals are warm-blooded animals and that we humans have a normal body temperature of about 37 \u00baC. Of course, at that age, we don’t really think much beyond that. The fact is, our body temperature actually changes throughout the day for a variety of reasons, and there is some very interesting control system biology going on to maintain body temperature in spite of large variations in the ambient temperature.<\/p>\n

This paper explores some of the biological mechanisms at the intersection of circadian body temperature variations and body temperature adaptation to environmental changes. Circadian clocks are manifested by a network of interconnected transcription factors and the numerous effectors under their control managing to produce a 24-hour oscillation of many cellular and organismic functions. Circadian changes in body temperature are well documented: the core temperature is warmest while awake and coldest when askeep.<\/p>\n

What they knew<\/strong><\/p>\n

Brown adipose tissue (BAT) is an important thermogenic (heat-producing) tissue in mammals, and that function is characterized by high glucose uptake (lots of fuel), oxidative capacity (“burning” the fuel), and mitochondrial uncoupling (using the released energy as heat instead of powering ATP formation). Rev-erb\u03b1\u00a0is a circadian transcriptional repressor (prevents expression of a particular gene or set of genes) that can regulate glucose and lipid metabolism in white adipose and other tissues, but its role, if any, in brown adipose tissue is unknown.<\/p>\n

What they did<\/strong><\/p>\n

\"cold1b\"<\/a>The first step was simply to see if Rev-erb\u03b1 plays any role in temperature regulation. To do this, the researchers compared the response of wild-type(wt<\/em>) and transgenic Rev-erb\u03b1 knockout(ko) mice when challenged with acute cold, lowering the temperature of their environment from 29 \u00baC (84 \u00baF) to 4 \u00baC (39 \u00baF) in thirty minutes. The figure at right shows that although both sets of mice experience a core body temperature drop to ~30 C within an hour, the core temp of the mutant mice then holds steady, while the wild-type mice experience further body temperature drop, which is reflected in the survival of the mice (by 3 hours, all rev-erb\u03b1 knockouts were alive, while half of the wild-type mice had died).<\/p>\n

\"cold1a\"<\/a>The interesting thing is that this difference only occurs when the experiment is run from Zeitgeber time (ZT, see vocabulary section above) 4:00-10:00. When the same experiment is run from ZT16-22, both wt<\/em> and ko<\/em> mice maintain a body temperature around 32 C, and there is 100% survival. This happens to coincide with a time-dependent drop in expression of Rev-erb\u03b1 in the wild-type mice (figure at left). The question is then, what does Rev-erb\u03b1 do? One clue could be in the oxygen consumption data: in the ZT 04:00-10:00 experiment, oxygen consumption ramped up strongly in the Rev-erb\u03b1 knockouts compared to wild-type (f, below). However, the oxygen is probably not being used by shivering muscles to generate heat (g). When the brown fat (BAT) was isolated from cold-treated animals though, there was a significant increase in oxygen consumption by the Rev-erb\u03b1 knockouts.<\/p>\n

\"cold1fgh\"<\/a><\/p>\n

We already saw that there is a normal rapid drop in Rev-erb\u03b1 expression every night in wt animals. Gerhart-Hines and his colleagues also noted that cold (4 \u00baC, blue bars, below) induced a strong drop in Rev-erba (left graph) in brown adipose tissue, compared to either normal temperature (29 \u00baC, red bars) or normal temperature with noradrenaline injection (white bars). They investigated the effect of noradrenaline because of a longstanding assumption that BAT thermogenesis is caused by release of the hormone noradrenaline, and activation of adrenergic signaling inside the BAT cells. There is a clear cold-induced increase in a nuclear receptor in the adrenergic signaling pathway (Nor1, right graph). There is also a cold-induced increase in Ucp1 (middle graph), which is an uncoupling protein, which as mentioned above, switches mitochondria from generating ATP to generating heat.<\/p>\n

\"cold2c\"<\/a><\/p>\n

In fact, Ucp1 levels rise very quickly as Rev-erb\u03b1 levels fall, suggesting that Rev-erba is a repressor of Ucp1. This is supported by the observation that even before cold-challenge, there is higher Ucp1 in the Rev-erba knockouts than in wild-type mice. In fact, when exogenous Rev-erb\u03b1 is introduced to Rev-erb\u03b1 knockouts, Ucp1 is reduced to wild-type levels (right).\"cold3d\"<\/a><\/p>\n

It seems clear that Rev-erb\u03b1 is shut down in response to cold so that brown fat tissue can start thermogenesis. It is not so clear why there is a circadian pattern to Rev-erb\u03b1 expression. One clue to that may be found in the an examination of core temperature in wt<\/em> and Rev-erb\u03b1\u00a0ko<\/em> mice. The normal night-time drop in body temperature (at normal ambient temperatures) is abolished in Rev-erb\u03b1\u00a0ko<\/em> mice.<\/p>\n

What it means<\/strong><\/p>\n

Brown adipose tissue likely evolved to allow mammals to survive a wide range of environmental temperatures. However, thermogenesis is extremely expensive to run, energetically (i.e. food) speaking. Therefore, a robust control mechanism likely evolved to balance the occasional need for thermogenesis and need to use food more efficiently in generating energy for the cells. The circadian rhythm of Rev-erb\u03b1 is useful because at night when it is low, the body is able to automatically respond to sudden unexpected changes in environmental temperature without the animal needing to be awake to react. It appears that Rev-erb\u03b1 is a temperature-dependent repressor of Ucp1, which controls thermogenesis in brown adipose tissue.<\/p>\n","protected":false},"excerpt":{"rendered":"

The paper: Gerhart-Hines, Z. et al. (2013) The nuclear receptor Rev-erb\u03b1 controls circadian thermogenic plasticity. Nature. doi:10.1038\/nature12642 Subject areas: Physiology, Cell Biology Vocabulary: Zeitgeber Time (ZT) – ZT is a convention used in chronobiology to standardize time effects on biological processes. In the experiments described here, the mice are acclimated […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"jetpack_post_was_ever_published":false,"_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","enabled":false},"version":2}},"categories":[7],"tags":[],"class_list":["post-457","post","type-post","status-publish","format-standard","hentry","category-bionews"],"jetpack_publicize_connections":[],"jetpack_featured_media_url":"","jetpack_sharing_enabled":true,"jetpack_shortlink":"https:\/\/wp.me\/p22HFc-7n","_links":{"self":[{"href":"https:\/\/www.axopub.com\/wp01\/wp-json\/wp\/v2\/posts\/457","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.axopub.com\/wp01\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.axopub.com\/wp01\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.axopub.com\/wp01\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.axopub.com\/wp01\/wp-json\/wp\/v2\/comments?post=457"}],"version-history":[{"count":3,"href":"https:\/\/www.axopub.com\/wp01\/wp-json\/wp\/v2\/posts\/457\/revisions"}],"predecessor-version":[{"id":466,"href":"https:\/\/www.axopub.com\/wp01\/wp-json\/wp\/v2\/posts\/457\/revisions\/466"}],"wp:attachment":[{"href":"https:\/\/www.axopub.com\/wp01\/wp-json\/wp\/v2\/media?parent=457"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.axopub.com\/wp01\/wp-json\/wp\/v2\/categories?post=457"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.axopub.com\/wp01\/wp-json\/wp\/v2\/tags?post=457"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}